E2F5 Antikörper (N-Term)

Produktnummer ABIN2792655

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch E2F5 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen und Zebrafisch (Danio rerio).

Kurzübersicht für E2F5 Antikörper (N-Term) (ABIN2792655)

Target: E2F5

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E2F5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-E2F5 Antikörper

Bindungsspezifität: N-Term

Sequenz: KAEIEDLELK ERELDQQKLL LQQSIKNVMD DSINNRFSYV THEDICNCFN

Kreuzreaktivität: Huhn, Human, Maus, Ratte (Rattus)

Homologie: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 84%

Produktmerkmale: This is a rabbit polyclonal antibody against E2F5. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human E2F5

Anwendungsinformationen

Applikationshinweise: WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 346 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Details zu E2F5

Target: E2F5

Andere Bezeichnung: E2F5

Hintergrund: E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
Alias Symbols: E2F-5
Protein Interaction Partner: RBL2, UBC, KMT2D, BRCC3, SMAD3, EP300, XPO1, CREBBP, TFDP1, RBL1, BIRC5, MYC, MT1G,
Protein Size: 346

Molekulargewicht: 38 kDa

Gen-ID: 1875

NCBI Accession: NM_001951, NP_001942

UniProt: Q15329